Part:BBa_K3285001:Design
Temperature sensitive repressor of cI(lambda) promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design was made by changing the 224th codon sequence from AAA to GAA.
Source
The part was constructed using the sequence suggested by the following paper:- "Amino acid changes in the repressor of bacteriophage lambda due to temperature-sensitive mutations in its cI gene and the structure of a highly temperature-sensitive mutant repressor." (Ref:- https://academic.oup.com/peds/article/12/3/225/1563912).
References
[1] Nandan Kumar Jana, Siddhartha Roy, Bhabatarak Bhattacharyya, Nitai Chandra Mandal, Amino acid changes in the repressor of bacteriophage lambda due to temperature-sensitive mutations in its cI gene and the structure of a highly temperature-sensitive mutant repressor, Protein Engineering, Design and Selection, Volume 12, Issue 3, March 1999, Pages 225–233, https://doi.org/10.1093/protein/12.3.225